Triton x-100 hemolysis
WebApr 13, 2024 · Then the supernatant obtained from suspension centrifugation was tested by reading the absorbance at 540 nm. 0.1% Triton X-100 was used as the positive control and saline as the negative control. The hemolysis ratio was … WebThe RBC suspension was incubated with Triton X-100 (10%, v/v) to obtain complete hemolysis. Two hours later, the samples were viewed under an optical microscope using a magnification of ×100. Then the RBC suspension was …
Triton x-100 hemolysis
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WebJun 1, 2002 · In the cases of both SDS and Triton X-100, there was a rather weak dependence of the HC 50 on the erythrocyte concentration; as a result, the amount of detergents per cell needed to cause 50%...
WebTemperature, °F Triton® X-405, 70% solution 40 2500 60 2000 80 575 100 250 120 125 Temperature, °C Weight Loss (2), % Triton® Surfactant 1 10 50 X-100 221 291 347 X-100 (N2 atmosphere) 210 310 350 Surface Tension, dynes/cm Active Ingredient, wt% Solvent Triton® Surfactant 1.0 0.1 0.01 0.001 Water X-114 29 29 30 44 X-100 30 30 31 46 WebUse of triton X-100 as a hemolytic agent in the spectrophotometric measurement of blood O2 saturation Use of triton X-100 as a hemolytic agent in the spectrophotometric measurement of blood O2 saturation J Appl Physiol. 1959 Jan;14(1):133-6.doi: 10.1152/jappl.1959.14.1.133. Authors G E DEIBLER, M S HOLMES, P L CAMPBELL, J GANS
WebTRITON™ X-100 Surfactant Product Information Chemical Description Name: Octylphenol Ethoxylate Surfactant Type: Nonionic O R O [ ]H x R=octyl(C8) x=9.5(avg) ... Actives, wt% 100 Cloud Point (1) 66 HLB (2) 13.4 Moles EO 9.5 pH, 5% aq solution 6 Pour Point(3) 1 Appearance(4) Pale yellow liquid WebTriton X-100 (positive control) Test Article Requirements 50 µL of 20 mM solution Analysis Method Spectophotometry at 540 nm Data Delivery Degree of lysis at each concentration …
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WebTriton X-100 to proteins. The 542 photometry Hungary). Results physiolog as con repeated potas did time treated lost The are In 75 was two of The determinations. deter incub of hemoglobfo (data X-100 to O .1 % he seconds. Discussion of cells under The potassium at character; half 75 respectively. hemoglo about minutes. release ... ghost handWebHemolysis assay: Triton™ X-100 (positive control). *** Statistically significant in comparison with the positive control (P < 0.05). Source publication Biopharmaceutical … front entry doors scottsdale azWebOct 17, 2014 · Hemolysis was expressed as the hemoglobin content obtained from the absorbance of the supernatant at 405 nm after centrifugation at 3000× g for 5 min. A 100% hemolysis was determined based on hemoglobin release after the addition of 0.1% Triton X-100. The hemolysis percentage of the peptides was calculated using the following formula: front entry doors for homes lowesWebOnly three detergents, Triton X-100, NP-40 and Brij-58, showed significant red blood cell lysis after 2% formaldehyde fixation for 10 min at room temperature. These detergents had a deleterious effect on whole blood cell light scatter at detergent concentration above 0.2%, whereas concentration below 0.1% resulted in incomplete red blood cell ... front entry doors glassWebMar 20, 2024 · The system was first tested using KCl saline solutions to achieve mass concentrations of 0.5%, 1%, 2%, and 4% in deionized water. This range of salinity was chosen to give changes in conductivity ... front entry doors new jerseyWebMay 23, 2002 · Only Loizaga et al. [11] reported that a Triton X-100/protein (4.7:1 w/v) was necessary to induce 50% hemolysis of human erythrocytes. The major objective of the present study was to quantitatively analyze the interaction of Triton X-100 with erythrocyte membranes using the approach described by Lichtenberg [23], [24]. front entry doors with sidelightWebJun 7, 2011 · Finally, the percent hemolysis was obtained by dividing each sample’s cell-free hemoglobin concentration by the total hemoglobin concentration (10 mg/ml). PEG (final concentration = 4.4%) was used as a negative control and Triton X-100 (final concentration = 1%) was used as a positive control. FIG. 1 Open in new tab Download slide ghost hand choke