Solid-phase synthesis of sirna
WebSolid-Phase Synthesis of Sequence-Defined T-, i-, and U-Shape Polymers for pDNA and siRNA Delivery ... have been used for the solid-phase-assisted synthesis of polymers with precise sequence, topology, and modifications (see picture). First proof-of-concept studies demonstrate the high potential of such polymers in pDNA and siRNA delivery. WebMar 1, 2008 · Assessment of 4-nitrogenated benzyloxymethyl groups for 2'-hydroxyl protection in solid-phase RNA synthesis. Cieślak J, Kauffman JS, Kolodziejski MJ, Lloyd …
Solid-phase synthesis of sirna
Did you know?
WebDec 8, 2024 · Beaucage SL (2008) Solid-phase synthesis of siRNA oligonucleotides. Curr Opin Drug Discov Dev 11:203–216. CAS Google Scholar Lönnberg H (2009) Solid-phase synthesis of oligonucleotide conjugates useful for delivery and targeting of potential nucleic acid therapeutics. Bioconjug Chem 20:1065–1094
WebNov 22, 2024 · A biolabile disulfide-containing linker (LSSL) was incorporated into siRNA strand at the final stage of solid-phase oligonucleotide synthesis through corresponding … WebDOI: 10.1016/J.CCLET.2013.06.010 Corpus ID: 97497625; Solid phase synthesis of peptide-siRNA conjugates containing disulfide bond unit @article{Wang2013SolidPS, title={Solid phase synthesis of peptide-siRNA conjugates containing disulfide bond unit}, author={Xiao-feng Wang and Xiantao Yang and Yue Chen and Yang Liu and Lang Zou and Zhenjun Yang …
WebApr 12, 2024 · This study describes the stereoselective synthesis of boranophosphate/phosphate (PB/PO) and phosphorothioate/phosphate (PS/PO) chimeric oligouridylates using the solid-phase metho Websolid-phase synthesis of RNA sequences, that is, that they should lead to phosphoramidite building blocks that are stable in solution and that also undergo relatively fast coupling reac-
WebSmall interfering RNA (siRNA) has been chemically conjugated to a variety of bioactive molecules, lipids, polymers, peptides, and inorganic nanostructured materials to enhance …
WebOlignucleotide-based drugs show promise as a novel form of chemotherapy. Among the hurdles that have to be overcome on the way of applicable nucleic acid therapeutics, … scan from brotherWebDec 13, 2007 · This protocol for solid-phase peptide synthesis (SPPS) is based on the widely used Fmoc/tBu strategy, activation of the carboxyl groups by aminium-derived … ruby create an arrayWebDNA Oligonucleotide Synthesis. Phosphoramidite chemistry, developed in the 1980s and later enhanced with solid-phase supports and automation, is the method of choice for … scan from bluetooth printerWebJul 4, 2008 · Request PDF Preparation of Zwitterionic Ribonucleoside Phosphoramidites for Solid-Phase siRNA Synthesis RNA oligomers, carrying 2'-O-modified nucleosides, proved to be extremely useful in ... scan from brother printer to dell computerWebApr 12, 2024 · The approval of Inclisiran – an siRNA containing the GalNAc moiety ... but the solid-phase process is neither readily scalable nor environmentally ... Many groups, including CatSci, are researching novel alternatives to solid-phase synthesis that can deliver an improved process with reduced waste, lower demand for acetonitrile, and ... scan from brother printer to computerWebAug 11, 2011 · Solid-Phase Synthesis of Sequence-Defined T-, i-, and U-Shape Polymers for pDNA and siRNA Delivery ... (ethylene amino) acids, together with selected natural amino … scan from brother printer to emailWebPreparation of the source siRNA transfection solution for solid-phase transfection 1. Prepare an siRNA stock solution by dissolving lyophilized siRNAs with milliQ water to a final concentration of 30 μM. 2. Transfer 3 μl of OptiMEM, containing 0.4 M sucrose to each well of a 384-well low-volume plate. 3. scan from brother mfc-l2710dw