Phic31 attp site
WebIsolated from the bacteriophage PhiC31, the PhiC31 integrase (frequently also written as: ΦC31 integrase) encodes a serine-type recombinase that mediates the sequence-specific … WebApr 1, 2004 · Abstract. The φC31 integrase functions efficiently in vitro and in Escherichia coli, yeast, and mammalian cells, mediating unidirectional site-specific recombination between its attB and attP recognition sites. Here we show that this site-specific integration system also functions efficiently in Drosophila melanogaster in cultured cells and in …
Phic31 attp site
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WebJan 2, 2024 · Specifically, the phiC31 integrase recognizes the attP sequence and introduces transgenes into this target site (Thorpe and Smith 1998 ). attP was introduced into the D. prolongata genome via piggyBac -mediated transgenesis and Kudo et al. ( 2024b) revealed the transformation efficiency and expression pattern in each strain with attP … WebThe phiC31 integrase system efficiently integrates plasmid DNA carrying a transgene and an attB site into a limited number of endogenous pseudo attP sites in mammalian genomes, leading to robust ...
WebThe Jump-In™ technology helps accelerate stable cell line development due to the targeted integration of genetic material into specific integration sites using PhiC31 integrase. Unlike the better-known recombinases such as Cre and Flp, PhiC31 integrase catalyzes recombination between two nonidentical sites and lacking a corresponding excision ... WebA method of controlling a genetically-modified plant, comprising (a) providing a genetically-modified plant, whereby cells of said genetically-modified plant contain a heterologous nucleic acid and whereby said genetically-modified plant is inactive with regard to a cellular process of interest, (b) switching on said cellular process of interest by directly …
WebPhiC31 Integrase Expression Plasmid Put the integrase into your PhiC31 and PinPoint genome editing projects with this vector that expresses PhiC31 Integrase for one-step single-copy transgenesis Non-viral transgene delivery Single-copy integration Preferential integration at active sites Delivery of inserts with no size constraints WebThe phiC31 (ΦC31) integrase system allows for site-specific integration of transgenes. Further, many attP sites have been characterized for position effects. The bacteriophage …
WebMay 7, 2013 · When phiC31-mediated site-specific integration occurs at the chromosomal attP site of the host strain, the whole sequence of the donor plasmid containing the attB site is inserted into the genome (Fig. 3a). To determine how such a reaction occurred in the established lines, we first performed inverse PCR and determined the flanking sequences.
WebJan 1, 2013 · Background: PhiC31 integrase is a DNA site-specific recombinase integrates DNA into the chromosomes between the two sites of attB and attP. Several pseudo attPs have been identified in... rick bayless new restaurant chicagoWebMay 5, 2013 · Background: PhiC31 integrase is a DNA site-specific recombinase integrates DNA into the chromosomes between the two sites of attB and attP. Several pseudo attPs have been identified in mammalian ... rick bayless pork loinWebWe injected the donor plasmid together with the phiC31 integrase mRNA into the embryos of the host strain and obtained positive lines. Structural analysis of the lines showed that site-specific integration occurred by recombination between the genomic attP site and the attB site of the donor plasmid. rick bayless pork tacosWebphiC31 integrase is a site-specific recombinase from a bacteriophage that has become a useful tool in mammalian cells. The enzyme normally performs precise, unidirectional … rick bayless net worth 2020WebSequences of PhiC31 and BxB1 attachment sites For each recombinase, the attB and attP attachment sites share a common central dinucleotide sequence (in red) that is used for the strand exchange... rick bayless on his brotherWebExpression of sgRNA under D. melanogaster U6-3 _(CR31539) promoter & delivery of D.mel-Actin5C::EGFP-2A-puro sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism Depositor Norbert Perrimon rick bayless pork tamalesWebJun 12, 2024 · The phage-derived phiC31 integrase is a useful tool for mediating sequence-specific genomic integration in mammalian cells, recombining donor plasmids bearing the attB recognition site with introduced genomic attP sites or endogeneous pseudo- attP sites having partial identity to attP. rick bayless recipes pork