2024 Hur antibody

Hur antibody

Author: rnmd

August undefined, 2024

WebHuR (Hu-antigen R, ELAV-like protein 1, ELAV1) is an RNA bindnig protein that contains 3 RNA-binding domains. It is known to binds AU-rich elements if mRNA. It regulates gene … WebWe determined that inhibitors of HuR (ELAVL1), a prosurvival mRNA stability factor that regulates cyclin D1, and an inhibitor of Yes …

RIPAb+ HuR - RIP Validated Antibody and Primer Set 03-102

WebImmunocytochemistry/ Immunofluorescence - Anti-HuR / ELAVL1 antibody (ab196626) Immunofluorscence analysis of MCF7 cells labeling HuR / ELAVL1 with ab196626 at 1/100 dilution (top panel). Blue: DAPI for nuclear staining (bottom panel). Western blot - Anti-HuR / ELAVL1 antibody (ab196626) WebRIP assays using anti-HuR antibody showed that ADORA2A-AS1 was specifically enriched in anti-HuR antibody group . These results demonstrated that ADORA2A-AS1 specifically bound to HuR. HuR is reported to bind and stabilize several mRNAs, including FSCN1, VEGFC, MAT2A, MDM2, CCND1, CASP3, USP7, FAS, PDGFA, CTSS, GATA3, and … fürdő vendéglő bük

ELAVL1 Gene - GeneCards ELAV1 Protein ELAV1 Antibody

WebHuR antibody (CL488-66549) Proteintech CoraLite® Plus 488-conjugated HuR Monoclonal antibody HuR Monoclonal Antibody for IF Host / Isotype Mouse / IgG2a Reactivity Human, mouse, rat Applications IF Conjugate CoraLite® Plus 488 Fluorescent Dye CloneNo. 1A11H2 Review Product Cat no : CL488-66549 Synonyms Webthat form complexes with HuR (see review [7]) are usually studied by ribonucleo-immunoprecipitation coupled to immunoblotting/RT-PCR or by RNA-Electrophoresis Mobility Shift Assays (REMSAs). However, these approaches have several limitations due to the necessity of good antibodies for immunoprecipitation and to the assumption that … WebELAVL1, also named as HUR, belongs to the RRM elav family. It is involved in 3'-UTR ARE-mediated MYC stabilization. ELAVL1 binds avidly to the AU-rich element in FOS and IL3/interleukin-3 mRNAs. In the case of the FOS AU-rich element, ELAVL1 binds to a … attijari bank devise tunisie

HuR antibody (11910-1-AP) Proteintech - ptglab

HuR antibody (CL594-11910) Proteintech - ptglab

WebProduct Details Product Specific Information This antibody detects pS, pT and pY amino acids with very low cross reactivity to their non-phosphorylated variants Target Information There are three amino acids … WebWe found that an mRNA-binding protein, human antigen R (HuR) associated with the 3′ UTR of AREG mRNA in response to UVB exposure, leading to enhanced mRNA stabilization. We also evaluated the … fürdő zala megyeWebDescription. HuR (Hu-antigen R, ELAV-like protein 1, ELAV1) is an RNA bindnig protein that contains 3 RNA-binding domains. It is known to binds AU-rich elements if mRNA. It regulates gene expression by destabilizing their mRNA. It is known to be upregulated in certain cancers such as breast and gastric cancers. attijari bank la goulette

"WebHuR and polypyrimidine tract binding protein (PTB) have been described as 'global regulators' of RNA alternative splicing, stability, and translation in non-neuronal (including ectodermal) tissues examined to date in diverse species, … " - Hur antibody

Hur antibody

HuR-hnRNP interactions and the effect of cellular stress

WebHuR inhibitor KH-3 is a small molecule, selective inhibitior of HuR function with Ki of 0.83 and 0.72 uM in FP and AlphaLISA assays, respectively. KH-3 potently inhibits breast cancer cell growth in vitro and in vivo. KH-3 inhibits breast cancer cell invasion in vitro as well as delays initiation of lung colonies and improves mouse survival in ... WebAll lanes : Anti-HuR / ELAVL1 antibody (ab28660) at 1 µg/ml Lane 1 : Wild-type SW480 cell lysate Lane 2 : ELAVL1 knockout SW480 cell lysate Lane 3 : HEK-293 cell lysate Lane 4 …

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Web16 feb. 2014 · Immunoprecipitation was performed using either anti-HuR or IgG control antibodies as described previously . Luciferase assay. PCR products corresponding to the 56-bp of WEE1 3′UTR and mutations in the 56-bp of WEE1 3′UTR region were purified and cloned into Promega psiCHECK2 luciferase vector (Promega). Web21 mrt. 2024 · HUR 3 4 5 ELAV (Embryonic Lethal, Abnormal Vision, Drosophila)-Like 1 (Hu Antigen R) 2 3 Embryonic Lethal, Abnormal Vision, Drosophila, Homolog-Like 1 2 3 …

WebAntibody Dilution Buffer (1X PBS / 1% BSA / 0.3% Triton X-100): To prepare 10 ml, add 30 µl Triton™ X-100 to 10 ml 1X PBS. Mix well then add 0.1 g BSA , mix. Recommended … Web25 However, although HuR acts as a positive regulator, CUGBP1 acts as a negative regulator of TJ/AJ proteins by competing with HuR for binding to the 3 0 UTR of occludin and E-cadherin. 22 In our ...

Web7 apr. 2024 · In 2015 and 2016, continued efforts to develop highly multiplexed FISH methods increased the number of detected genes from a dozen to several hundred and then up to one thousand transcripts per cell [multiplexed error-robust FISH (MERFISH) [] and sequential FISH (seqFISH), [] Figure 1a,b].To encode as many transcripts as possible, … WebPublished figure using HuR monoclonal antibody (Product # A-21277) in Immunohistochemistry Figure 3 Knockdown of IGF2BPs decreases mRNA stability …

WebHuR Polyclonal antibody: Catalog Number: CL594-11910 Citations-Dilutions: Applications: FC (Intra) Reactivity: human, mouse, rat Product Guarantee: Covers any species …

Web2 dagen geleden · This article aims to develop a novel highly sensitive paper-based immunoassay. Here in, the paper zones were modified with GO and pyrene derivatives, the antibody immobilization strategy was illustrated in Scheme 1.Under optimal pH, Fc fragments of antibodies were pre-adsorbed onto GO/pyrene modified cellulose surface … fürdő étterem bükfürdőWebInvitrogen Anti-HuR Monoclonal (3A2), Catalog # MA1-167. Tested in Western Blot (WB), Immunocytochemistry (ICC/IF), Immunoprecipitation (IP) and RNA Immunoprecipitation … attijari bank net tunisieWebArticle Snippet: Samples were then analyzed by western blot using the desired antibodies, anti-SARS-CoV-2 N protein (40143-MM05, Sino Biological), anti-HuR antibody (3A2, Santa Cruz), anti-Myc tag antibody (ab9106) or anti-Ace-2 antibody (ab15348) followed by the respective secondary antibodies (horseradish peroxidase-conjugated anti-mouse or anti … fürdő étterem bükfürdő etlapWeb1 dag geleden · The following primary antibodies were used for immunoblotting: antibodies against HA Tag (3724, CST), HuR (2582, CST), and pERK1/2 (4370, CST) were used at 1:1,000 dilution, and antibodies against Vinculin (13901, CST) and Tubulin (T6199, Sigma) were used at 1:3,000 dilution as the internal controls. fürdő étterem bük eladóWeb30 mrt. 2015 · RNA binding protein (RBPs) and microRNAs (miRNAs or miRs) are post-transcriptional regulators of gene expression that are implicated in development of cancers. Although their individual roles have been studied, the crosstalk between RBPs and miRNAs is under intense investigation. Here, we show that in breast cancer cells, cyclin E1 … attijari bank sahloul hammam sousseWebHuR antibody (CL488-66549) Proteintech CoraLite® Plus 488-conjugated HuR Monoclonal antibody HuR Monoclonal Antibody for IF Host / Isotype Mouse / IgG2a … fürdő étterem bük étlapWeb28 dec. 2024 · Input is whole cell lysate. IgG is the IP with the control antibody and HuR refers to IP with the anti-HuR antibody. Note that the enrichment of β-actin (b), ACTA2 (c), COL1A1 (d) and FN (e) is unaffected by exposure to hypoxia. Values are represented as the mean ± SEM (n = 4 independent experiments) attijari bank sousse sahloul