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OsSWEET14 cooperates with OsSWEET11 to contribute to …
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Plasma membrane-localized SlSWEET7a and SlSWEET14 regulate
WebApr 4, 2005 · The EBY.VW 4000 strain is able to grow on maltose, but not on glucose or fructose (Wieczorke et al., 1999). Complementation by pDR195-VvHT1 restores growth on 100 μM glucose ( Fig. 1A ). Uptake experiments with labelled sugars showed that VvHT1 mediates the uptake of [ 14 C] glucose with high affinity, whereas no glucose uptake was … WebThe hxt0 yeast strain EBY.VW4000 is widely used for characterization and screening of sugar transporters. De-letion of all hexose transporter genes in this strain prevents uptake of glucose and xylose and allows manipulation and assessment of distinct, reintroduced transporters. This strain was the parental strain to our screening strain. WebComplementation of yeast EBY. VW4000 mutant by MeSTP genes. Yeast cell suspensions with optical density (OD) at 600 nm of 0.6 and three corresponding 1:10 dilutions were dropped on solid SD-ura ... churchbase login