WebJun 7, 2016 · Genomic DNA (1.5 ug) was fragmented using a Covaris S2 focused ultrasonicator in micro TUBE AFA Fiber Pre-Slit Snap-Cap 6×16 mm micro tubes and the Covaris MicroTUBE holder (covaris part numbers ... WebDNA Shearing with S220 Focused-ultrasonicator . This Quick Guide provides DNA Shearing protocols when using microTUBE-130, microTUBE-50, microTUBE-15, microTUBE-500, or miniTUBE and a Covaris S220 Focused-ultrasonicator. Revision History Part Number Revision Date Description of change 010368 C 12/16 Correct PIP …
Covaris S2 Focused Ultrasonicator Unit4 Pred S220
WebTo fragment DNA to size larger than 5 kb, Covaris offers the g-TUBE: a single-use device that shears genomic DNA into selected fragments sizes ranging from 6 kb to 20 kb. The only equipment needed is a compatible bench-top centrifuge. Values mentioned in this Quick Guide are nominal values. The tolerances are as follow: - Temperature +/-2°C WebThe Covaris S220 Focused-ultrasonicator is a high-powered system that uses controlled Adaptive Focused Acoustic (AFA) Ultrasonic Technology for shearing DNA, RNA or chromatin to specific fragment lengths in sample volumes ranging from 25 µl to 10 ml. The system can also be used for tissue sonication. lego star wars 3 gold brick cheat engine
S-series
WebJun 6, 2024 · The Covaris S2 focused-ultrasonicator is a versatile, high power system engineered for pre-analytical sample processing for numerous biological and chemical applications including, DNA, RNA, and chromatin shearing, tissue homogenization, cell lysis, compound dissolution, and particle micronization. WebMar 7, 2024 · Library preparation protocols varied depending on DNA quality. For higher-quality extractions (i.e., average fragment size > 350 bp), DNA was sonicated in an M220 Focused-ultrasonicator with microTUBES AFA Fiber Pre-slit Snap-caps (Covaris, Massachusetts, USA) following the manufacturer’s protocol. WebApr 11, 2015 · Background: Fragmentation at random nucleotide locations is an essential process for preparation of DNA libraries to be used on massively parallel short-read DNA sequencing platforms. Although instruments for physical shearing, such as the Covaris S2 focused-ultrasonicator system, and products for enzymatic shearing, such as the … lego star wars 3 mac torrent